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Western blot All lanes: CFH antibody at 3microg/ml + human serum Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 140, 52 kDa Observed band size: 140 kDa
Western blot All lanes: CFH antibody at 3microg/ml + human serum Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 140, 52 kDa Observed band size: 140 kDa
Western blot All lanes: CFH antibody at 3microg/ml + human serum Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 140, 52 kDa Observed band size: 140 kDa

CFH Antibody

CSB-PA005273ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCFH
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Overview

  • Supplier
    Cusabio
  • Product Name
    CFH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3075
  • Target name
    CFH
  • Target description
    complement factor H
  • Target synonyms
    adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin; CFHL3; complement factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 2 (complement); HF; HF1; HF2; HUS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08603
  • Protein Name
    Complement factor H
  • Scientific Description
    Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor H/CFH using anti-Factor H/CFH antibody (A00562-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor H/CFH antigen affinity purified polyclonal antibody (Catalog # A00562-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor H/CFH at approximately 150 kDa. The expected band size for Factor H/CFH is at 139 kDa.
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