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Immunohistochemical staining of human spleen shows weak membranous positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows weak membranous positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows weak membranous positivity in cells in red pulp.

Anti-FCGRT Antibody

HPA015130
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetFCGRT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FCGRT
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2217
  • Target name
    FCGRT
  • Target description
    Fc fragment of IgG receptor and transporter
  • Target synonyms
    alpha-chain; Fc fragment of IgG, receptor, transporter, alpha; FCRN; FcRn alpha chain; heavy chain of the major histocompatibility complex class I-like Fc receptor; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; neonatal Fc receptor; neonatal Fc-receptor for Ig; transmembrane alpha chain of the neonatal receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55899
  • Protein Name
    IgG receptor FcRn large subunit p51
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: FCGRT antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
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Figure 1. Western blot analysis of FCGRT using anti-FCGRT antibody (PB9866). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGRT antigen affinity purified polyclonal antibody (Catalog # PB9866) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGRT at approximately 50 kDa. The expected band size for FCGRT is at 40 kDa.
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