Figure 1. Western blot analysis of FENS1/WDFY1 using anti-FENS1/WDFY1 antibody (A10318-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FENS1/WDFY1 antigen affinity purified polyclonal antibody (Catalog # A10318-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FENS1/WDFY1 at approximately 46 kDa. The expected band size for FENS1/WDFY1 is at 46 kDa.