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ProductsBack/WDFY1 + WDFY2 antibody [HL1989]
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WDFY1+WDFY2 antibody [HL1989] (GTX637890) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WDFY1+WDFY2 antibody [HL1989] (GTX637890) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WDFY1+WDFY2 antibody [HL1989] (GTX637890) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WDFY1 + WDFY2 antibody [HL1989]

GTX637890
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetWDFY1
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Overview

  • Supplier
    GeneTex
  • Product Name
    WDFY1 + WDFY2 antibody [HL1989]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1989
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57590
  • Target name
    WDFY1
  • Target description
    WD repeat and FYVE domain containing 1
  • Target synonyms
    FENS-1, FENS1, WDF1, ZFYVE17, WD repeat and FYVE domain-containing protein 1, FYVE domain-containing protein localized to endosomes 1, WD40- and FYVE domain-containing protein 1, phosphoinositide-binding protein SR1, zinc finger FYVE domain-containing protein 17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWB7
  • Protein Name
    WD repeat and FYVE domain-containing protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of FENS1/WDFY1 using anti-FENS1/WDFY1 antibody (A10318-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FENS1/WDFY1 antigen affinity purified polyclonal antibody (Catalog # A10318-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FENS1/WDFY1 at approximately 46 kDa. The expected band size for FENS1/WDFY1 is at 46 kDa.
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