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Immunohistochemical staining of human gallbladder shows strong granular positivity in cytoplasm in glandular cells.
Immunohistochemical staining of human gallbladder shows strong granular positivity in cytoplasm in glandular cells.
Immunohistochemical staining of human gallbladder shows strong granular positivity in cytoplasm in glandular cells.

Anti-FGF19 Antibody

HPA036082
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetFGF19
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FGF19
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9965
  • Target name
    FGF19
  • Target description
    fibroblast growth factor 19
  • Target synonyms
    FGF-19; fibroblast growth factor 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95750
  • Protein Name
    Fibroblast growth factor 19
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: 293T whole cell lysate All lanes: FGF19 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
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Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (PB10061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (Catalog # PB10061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
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