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WB analysis of HeLa (A), mouse liver (B), PC3 (C) whole cell lysates using GTX56182 FGF19 antibody.
WB analysis of HeLa (A), mouse liver (B), PC3 (C) whole cell lysates using GTX56182 FGF19 antibody.
WB analysis of HeLa (A), mouse liver (B), PC3 (C) whole cell lysates using GTX56182 FGF19 antibody.

FGF19 antibody

GTX56182
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetFGF19
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Overview

  • Supplier
    GeneTex
  • Product Name
    FGF19 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9965
  • Target name
    FGF19
  • Target description
    fibroblast growth factor 19
  • Target synonyms
    FGF-19; fibroblast growth factor 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95750
  • Protein Name
    Fibroblast growth factor 19
  • Scientific Description
    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (PB10061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (Catalog # PB10061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
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