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Western Blot Positive WB detected in: 293T whole cell lysate All lanes: FGF19 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: FGF19 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: FGF19 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa

FGF19 Antibody

CSB-PA008624EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFGF19
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Overview

  • Supplier
    Cusabio
  • Product Name
    FGF19 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9965
  • Target name
    FGF19
  • Target description
    fibroblast growth factor 19
  • Target synonyms
    FGF-19; fibroblast growth factor 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95750
  • Protein Name
    Fibroblast growth factor 19
  • Scientific Description
    Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (PB10061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (Catalog # PB10061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
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