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Immunohistochemical staining of human caudate shows nuclear positivity in glia.
Immunohistochemical staining of human caudate shows nuclear positivity in glia.
Immunohistochemical staining of human caudate shows nuclear positivity in glia.

Anti-FGF2 Antibody

HPA065502
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetFGF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FGF2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2247
  • Target name
    FGF2
  • Target description
    fibroblast growth factor 2
  • Target synonyms
    basic fibroblast growth factor bFGF; BFGF; FGF-2; FGFB; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; prostatropin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09038
  • Protein Name
    Fibroblast growth factor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SKOV3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
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