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FGF2 / Basic FGF Antibody (Preservative Free)

LS-C343397
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFGF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FGF2 / Basic FGF Antibody (Preservative Free)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2247
  • Target name
    FGF2
  • Target description
    fibroblast growth factor 2
  • Target synonyms
    BFGF, FGF-2, FGFB, HBGF-2, fibroblast growth factor 2, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2, prostatropin
  • Host
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SKOV3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
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