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Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

FGF2 Polyclonal Antibody

BS-0217R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFGF2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FGF2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2247
  • Target name
    FGF2
  • Target description
    fibroblast growth factor 2
  • Target synonyms
    basic fibroblast growth factor bFGF; BFGF; FGF-2; FGFB; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; prostatropin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09038
  • Protein Name
    Fibroblast growth factor 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Tort S et al., 2020 Feb, Burns
    Read more
  • Expression of osteoprotegerin and receptor activator for the nuclear factor-kappaB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis. Peng W et al., 2018 Oct 18, J Cell Biochem
    Read more
  • Effects of l-arginine on endometrial microvessel density in nutrient-restricted Hu sheep. Gao XX et al., 2018 Oct 1, Theriogenology
    Read more
  • B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model. Liu G et al., 2018 Jul, Eye (Lond)
    Read more
  • VEGF and BFGF Expression and Histological Characteristics of the Bone-Tendon Junction during Acute Injury Healing. Wang L et al., 2014 Jan, J Sports Sci Med
    Read more

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Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SKOV3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
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