Overlay histogram showing U-2OS cells stained with M10171 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M10171, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was mouse IgG2a (1microg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.