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Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or mouse FLIPL (lane 3) expression plasmid using anti-FLIP, mAb (Dave-2) (AG-20B-0005). Untransfected cells (lane 1). Top arrows indicate full length FLIP, lower
Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or mouse FLIPL (lane 3) expression plasmid using anti-FLIP, mAb (Dave-2) (AG-20B-0005). Untransfected cells (lane 1). Top arrows indicate full length FLIP, lower
Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or mouse FLIPL (lane 3) expression plasmid using anti-FLIP, mAb (Dave-2) (AG-20B-0005). Untransfected cells (lane 1). Top arrows indicate full length FLIP, lower

anti-FLIP, mAb (Dave-2)

AG-20B-0005
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetCFLAR
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-FLIP, mAb (Dave-2)
  • Delivery Days Customer
    10
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Dave-2
  • Concentration
    1 mg/ml
  • Gene ID8837
  • Target name
    CFLAR
  • Target description
    CASP8 and FADD like apoptosis regulator
  • Target synonyms
    CASH; CASP8 and FADD-like apoptosis regulator; CASP8AP1; caspase homolog; caspase-eight-related protein; caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; Casper; cellular FLICE-like inhibitory protein; cFLIP; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CLARP; FADD-like antiapoptotic molecule 1; FLAME; FLAME1; FLAME-1; FLIP; I-FLICE; inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; testis secretory sperm-binding protein Li 233m; usurpin beta
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDO15519
  • Protein Name
    CASP8 and FADD-like apoptosis regulator
  • Scientific Description
    FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes. - Monoclonal Antibody. Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP. Isotype: Rat IgG2a. Clone: Dave-2. Applications: IP, WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FLIP using anti-FLIP antibody (A01295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates, Lane 7: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLIP antigen affinity purified polyclonal antibody (Catalog # A01295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLIP at approximately 43KD. The expected band size for FLIP is at 55KD.
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