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Immunohistochemical staining of human placenta shows cytoplasmic positivity in endothelial cells and trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in endothelial cells and trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in endothelial cells and trophoblastic cells.

Anti-FLT4 Antibody

HPA074389
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFLT4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FLT4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2324
  • Target name
    FLT4
  • Target description
    fms related receptor tyrosine kinase 4
  • Target synonyms
    CHTD7, FLT-4, FLT41, LMPH1A, LMPHM1, PCL, VEGFR-3, VEGFR3, vascular endothelial growth factor receptor 3, Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4, VEGF receptor-3, fms related tyrosine kinase 4, fms-like tyrosine kinase 4, primary congenital lymphedema, tyrosine-protein kinase receptor FLT4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35916
  • Protein Name
    Vascular endothelial growth factor receptor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 3 antigen affinity purified polyclonal antibody (Catalog # A01276-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 3 at approximately 153KD. The expected band size for VEGF Receptor 3 is at 153KD.
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