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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Anti-FMO1 Antibody

HPA078132
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFMO1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FMO1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2326
  • Target name
    FMO1
  • Target description
    flavin containing dimethylaniline monoxygenase 1
  • Target synonyms
    flavin-containing monooxygenase 1, FMO 1, Flavin-containing monooxygenase 1 (fetal liver), dimethylaniline monooxygenase [N-oxide-forming] 1, dimethylaniline oxidase 1, fetal hepatic flavin-containing monooxygenase 1, flavin containing monooxygenase 1, trimethylamine monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01740
  • Protein Name
    Dimethylaniline monooxygenase [N-oxide-forming] 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FMO1 using anti-FMO1 antibody (PB9589). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: Rat Kidney Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO1 antigen affinity purified polyclonal antibody (Catalog # PB9589) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO1 at approximately 60 kDa. The expected band size for FMO1 is at 60 kDa.
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