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ProductsBack/Anti-FNDC5 (-C) (94-111) Antibody

Anti-FNDC5 (-C) (94-111) Antibody

130-10570
RayBiotech
ApplicationsELISA
Product group Antibodies
TargetFNDC5
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-FNDC5 (-C) (94-111) Antibody
  • Delivery Days Customer
    16
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID252995
  • Target name
    FNDC5
  • Target description
    fibronectin type III domain containing 5
  • Target synonyms
    FRCP2, irisin, fibronectin type III domain-containing protein 5, fibronectin type III repeat-containing protein 2
  • Host
    Rabbit
  • Protein IDQ8NAU1
  • Protein Name
    Fibronectin type III domain-containing protein 5
  • Scientific Description
    Rabbit anti-Human FNDC5 (-C)(94-111) Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.
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Lane 1: HepG2 cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse heart lysates probed with FNDC5 Polyclonal Antibody, Unconjugated (bs-8486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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