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Fibronectin Type III Domain Containing Protein 5 (FNDC5) Polyclonal Antibody

CAU21253
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Porcine, Rat
TargetFNDC5
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Overview

  • Supplier
    Biomatik
  • Product Name
    Fibronectin Type III Domain Containing Protein 5 (FNDC5) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID252995
  • Target name
    FNDC5
  • Target description
    fibronectin type III domain containing 5
  • Target synonyms
    FRCP2, irisin, fibronectin type III domain-containing protein 5, fibronectin type III repeat-containing protein 2
  • Host
    Rabbit
  • Protein IDQ8NAU1
  • Protein Name
    Fibronectin type III domain-containing protein 5
  • Scientific Description
    The Fibronectin Type III Domain Containing Protein 5 (FNDC5) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Porcine, Rat
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.
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Lane 1: HepG2 cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse heart lysates probed with FNDC5 Polyclonal Antibody, Unconjugated (bs-8486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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WB analysis of various sample lysates using GTX03463 FNDC5 antibody. Lane 1 : HepG2 whole cell lysate Lane 2 : NIH/3T3 whole cell lysate Lane 3 : K562 whole cell lysate Lane 4 : Mouse heart tissue lysate Dilution : 1:1000
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IHC image of CSB-PA836707OA01HU diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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