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Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Anti-FNDC5 Antibody

HPA051290
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetFNDC5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FNDC5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID252995
  • Target name
    FNDC5
  • Target description
    fibronectin type III domain containing 5
  • Target synonyms
    fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2; FRCP2; irisin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NAU1
  • Protein Name
    Fibronectin type III domain-containing protein 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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WB analysis of various sample lysates using GTX03463 FNDC5 antibody. Lane 1 : HepG2 whole cell lysate Lane 2 : NIH/3T3 whole cell lysate Lane 3 : K562 whole cell lysate Lane 4 : Mouse heart tissue lysate Dilution : 1:1000
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Lane 1: HepG2 cell lysates; Lane 2: NIH/3T3 cell lysates; Lane 3: K562 cell lysates; Lane 4: Mouse heart lysates probed with FNDC5 Polyclonal Antibody, Unconjugated (bs-8486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.
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