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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.

Anti-FOXA3 Antibody

HPA054034
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetFOXA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FOXA3
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3171
  • Target name
    FOXA3
  • Target description
    forkhead box A3
  • Target synonyms
    FKHH3, HNF3G, TCF3G, hepatocyte nuclear factor 3-gamma, HNF-3-gamma, HNF-3G, TCF-3G, fork head-related protein FKH H3, forkhead box protein A3, transcription factor 3G
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55318
  • Protein Name
    Hepatocyte nuclear factor 3-gamma
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of FOXA3 using anti-FOXA3 antibody (PB9805). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA3 antigen affinity purified polyclonal antibody (Catalog # PB9805) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA3 at approximately 40 kDa. The expected band size for FOXA3 is at 37 kDa.
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WB analysis of various samples using GTX04941 FOXA3 antibody. Lane 1 : Rat liver tissue lysate (50 microg) Lane 2 : Rat pancreas tissue lysate (50 microg) Lane 3 : Mouse liver tissue lysate (50 microg) Lane 4 : HeLa whole cell lysate (40 microg) Dilution : 0.5 microg/mL
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