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FOXA3 Antibody

LS-C680489
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFOXA3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FOXA3 Antibody
  • Delivery Days Customer
    14
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3171
  • Target name
    FOXA3
  • Target description
    forkhead box A3
  • Target synonyms
    FKHH3, HNF3G, TCF3G, hepatocyte nuclear factor 3-gamma, HNF-3-gamma, HNF-3G, TCF-3G, fork head-related protein FKH H3, forkhead box protein A3, transcription factor 3G
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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IHC image of CSB-PA008796LA01HU diluted at 1:700 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of FOXA3 using anti-FOXA3 antibody (PB9805). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA3 antigen affinity purified polyclonal antibody (Catalog # PB9805) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA3 at approximately 40 kDa. The expected band size for FOXA3 is at 37 kDa.
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TargetFOXA3
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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
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WB analysis of various samples using GTX04941 FOXA3 antibody. Lane 1 : Rat liver tissue lysate (50 microg) Lane 2 : Rat pancreas tissue lysate (50 microg) Lane 3 : Mouse liver tissue lysate (50 microg) Lane 4 : HeLa whole cell lysate (40 microg) Dilution : 0.5 microg/mL
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