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ProductsBack/anti-FOXP3 antibody [SP97]

anti-FOXP3 antibody [SP97]

ARG53050
Arigo Biolaboratories
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetFOXP3
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-FOXP3 antibody [SP97]
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubation Time: 20 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SP97
  • Conjugate
    Unconjugated
  • Gene ID50943
  • Target name
    FOXP3
  • Target description
    forkhead box P3
  • Target synonyms
    AIID, DIETER, IPEX, JM2, PIDX, XPID, forkhead box protein P3, FOXP3delta7, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, immunodeficiency, polyendocrinopathy, enteropathy, X-linked
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZS1
  • Protein Name
    Forkhead box protein P3
  • Scientific Description
    The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXP3 using anti-FOXP3 antibody (A00011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP3 antigen affinity purified polyclonal antibody (Catalog # A00011-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP3 at approximately 47 kDa. The expected band size for FOXP3 is at 47 kDa.
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