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Western blot analysis using anti-FOXP3 (mouse), pAb (Prod. No. AG-25A-0020) at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treate
Western blot analysis using anti-FOXP3 (mouse), pAb (Prod. No. AG-25A-0020) at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treate
Western blot analysis using anti-FOXP3 (mouse), pAb (Prod. No. AG-25A-0020) at 1:3000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Mouse T lymphocyte (CD4+) cell lysate. 4: PHA treate

anti-FOXP3 (mouse), pAb

AG-25A-0020
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFoxp3
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-FOXP3 (mouse), pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID20371
  • Target name
    Foxp3
  • Target description
    forkhead box P3
  • Target synonyms
    forkhead box protein P3; JM2; scu; scurfin; scurfy; sf
  • Host
    Rat
  • Protein IDQ99JB6
  • Protein Name
    Forkhead box protein P3
  • Scientific Description
    FOXP3 is involved in immune system responses. It functions as the master regulator in the development and function of regulatory T cells. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX); also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. - Polyclonal Antibody. Recognizes mouse FOXP3. Detects a band of ~46-49kDa by Western blot. Source: Rat. Applications: ELISA, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. FOXP3 is involved in immune system responses. It functions as the master regulator in the development and function of regulatory T cells. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX); also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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Western blot analysis using anti-FOXP3 (mouse), pAb (Prod. No. AG-25A-0035) at 1:5000 dilution. 1: Mouse FOXP3 (His-tagged). 2: Transfected mouse FOXP3 cell lysate (HEK 293). 3: Transfected vector only cell lysate.
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