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Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.

Anti-FSCN2 Antibody

HPA079270
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetFSCN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FSCN2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25794
  • Target name
    FSCN2
  • Target description
    fascin actin-bundling protein 2, retinal
  • Target synonyms
    fascin homolog 2, actin-bundling protein, retinal; fascin-2; RFSN; RP30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14926
  • Protein Name
    Fascin-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FSCN2 using anti-FSCN2 antibody (A07840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSCN2 antigen affinity purified polyclonal antibody (Catalog # A07840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSCN2 at approximately 60KD. The expected band size for FSCN2 is at 55KD.
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Product group Antibodies
References
Everest Biotech
Goat anti-Fascin 2EB08002
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Atlas Antibodies
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