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Goat anti-Fascin 2 Antibody

EB08002
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetFSCN2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Fascin 2 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID25794
  • Target name
    FSCN2
  • Target description
    fascin actin-bundling protein 2, retinal
  • Target synonyms
    RFSN, RP30, fascin-2, fascin homolog 2, actin-bundling protein, retinal
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001070650.1; NP_036550.1. GeneIDs all Nonhuman: 238021 (mouse); 303741 (rat);. Purification: Antigen affinity purified. Names and symbols: fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus); RFSN; RP30; fascin 2; retinal fascin;
  • Reactivity
    Canine, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ramakrishnan NA, Drescher MJ, Khan KM, et al. HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2. J Biol Chem. 2012,287(45):37628-46. doi: 10.1074/jbc.M112.375832
    Read this paper
  • Shin JB, Longo-Guess CM, Gagnon LH, et al. The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice. J Neurosci. 2010,30(29):9683-94. doi: 10.1523/JNEUROSCI.1541-10.2010
    Read this paper

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Figure 1. Western blot analysis of FSCN2 using anti-FSCN2 antibody (A07840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSCN2 antigen affinity purified polyclonal antibody (Catalog # A07840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSCN2 at approximately 60KD. The expected band size for FSCN2 is at 55KD.
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