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Fascin 2 (FSCN2) Polyclonal Antibody

CAU22753
Biomatik
ApplicationsWestern Blot
Product group Antibodies
TargetFSCN2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Fascin 2 (FSCN2) Polyclonal Antibod
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25794
  • Target name
    FSCN2
  • Target description
    fascin actin-bundling protein 2, retinal
  • Target synonyms
    RFSN, RP30, fascin-2, fascin homolog 2, actin-bundling protein, retinal
  • Host
    Rabbit
  • Protein IDO14926
  • Protein Name
    Fascin-2
  • Scientific Description
    The Fascin 2 (FSCN2) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FSCN2 using anti-FSCN2 antibody (A07840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSCN2 antigen affinity purified polyclonal antibody (Catalog # A07840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSCN2 at approximately 60KD. The expected band size for FSCN2 is at 55KD.
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Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor cells.
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