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Anti-FURIN Antibody

144-62750
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetFURIN
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-FURIN Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID5045
  • Target name
    FURIN
  • Target description
    furin, paired basic amino acid cleaving enzyme
  • Target synonyms
    FUR, PACE, PCSK3, SPC1, furin, FES upstream region, dibasic processing enzyme, furin, membrane associated receptor protein, paired basic amino acid residue-cleaving enzyme, proprotein convertase subtilisin/kexin 3, proprotein convertase subtilisin/kexin type 3, subtilisin-like proprotein convertase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09958
  • Protein Name
    Furin
  • Scientific Description
    FURIN pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Furin using anti-Furin antibody (PB9182). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Furin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 40 kDa. The expected band size for Furin is at 40 kDa.
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Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Hela Cells; Lane 4: MCF-7 Cells; Probed with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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