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ProductsBack/Anti-Galectin-3 APC

Anti-Galectin-3 APC

1A-876
EXBIO
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-Galectin-3 APC
  • Delivery Days Customer
    7
  • Application Supplier Note
    Flow cytometry: Recommended dilution: 1-5 microg/ml.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clone ID
    M3/38
  • Concentration
    0.1 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Isotype
    IgG2a
  • Scientific Description
    Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # PB9279) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3/LGALS3 at approximately 29 kDa. The expected band size for Galectin 3/LGALS3 is at 29 kDa.
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