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Western blot analysis of GART expression in HeLa cell lysate.
Western blot analysis of GART expression in HeLa cell lysate.
Western blot analysis of GART expression in HeLa cell lysate.

Anti-GART Rabbit Monoclonal Antibody

M06061
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetGART
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GART Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FFD-7
  • Gene ID2618
  • Target name
    GART
  • Target description
    phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
  • Target synonyms
    AIRS; GAR transformylase; GARS; GARS-AIRS-GART; GARTF; glycinamide ribonucleotide formyltransferase; glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase; PAIS; PGFT; PRGS; trifunctional purine biosynthetic protein adenosine-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22102
  • Protein Name
    Trifunctional purine biosynthetic protein adenosine-3
  • Scientific Description
    Boster Bio Anti-GART Rabbit Monoclonal Antibody catalog # M06061. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GART using anti-GART antibody (A06061-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GART antigen affinity purified polyclonal antibody (Catalog # A06061-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GART at approximately 108 kDa. The expected band size for GART is at 108 kDa.
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