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Immunohistochemistry analysis in human bone marrow and pancreas tissues using HPA000232 antibody. Corresponding GATA1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human bone marrow and pancreas tissues using HPA000232 antibody. Corresponding GATA1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human bone marrow and pancreas tissues using HPA000232 antibody. Corresponding GATA1 RNA-seq data are presented for the same tissues.

Anti-GATA1-25ul

HPA000232
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GATA1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Applications Supplier
    WB, ChIP, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15976
  • Protein Name
    Erythroid transcription factor
  • Scientific Description
    Rabbit Polyclonal Anti-GATA1 Antibody against Human GATA binding protein 1 (globin transcription factor 1). Validated for Chromatin Immunoprecipitation, Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GATA1 using anti-GATA1 antibody (A00408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 antigen affinity purified polyclonal antibody (Catalog # A00408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA1 at approximately 45KD. The expected band size for GATA1 is at 45KD.
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