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Goat anti-GATA1

EB05571
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetGATA1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GATA1 Antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2623
  • Target name
    GATA1
  • Target description
    GATA binding protein 1
  • Target synonyms
    ERYF1; erythroid transcription factor; erythroid transcription factor 1; GATA-1; GATA-binding factor 1; GF1; GF-1; globin transcription factor 1; NFE1; NF-E1; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1; XLANP; XLTDA; XLTT
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GATA1 using anti-GATA1 antibody (A00408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 antigen affinity purified polyclonal antibody (Catalog # A00408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA1 at approximately 45KD. The expected band size for GATA1 is at 45KD.
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