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Mouse spleen lysates probed with Rabbit Anti-GATA-1 Polyclonal Antibody (bs-3872R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band size: 35,45kDa and observed band size: 35kDa
Mouse spleen lysates probed with Rabbit Anti-GATA-1 Polyclonal Antibody (bs-3872R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band size: 35,45kDa and observed band size: 35kDa
Mouse spleen lysates probed with Rabbit Anti-GATA-1 Polyclonal Antibody (bs-3872R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band size: 35,45kDa and observed band size: 35kDa

GATA1 Polyclonal Antibody

BS-3872R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGATA1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GATA-1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2623
  • Target name
    GATA1
  • Target description
    GATA binding protein 1
  • Target synonyms
    ERYF1; erythroid transcription factor; erythroid transcription factor 1; GATA-1; GATA-binding factor 1; GF1; GF-1; globin transcription factor 1; NFE1; NF-E1; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1; XLANP; XLTDA; XLTT
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15976
  • Protein Name
    Erythroid transcription factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GATA1 using anti-GATA1 antibody (A00408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 antigen affinity purified polyclonal antibody (Catalog # A00408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA1 at approximately 45KD. The expected band size for GATA1 is at 45KD.
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