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ProductsBack/Anti-GC Antibody

Anti-GC Antibody

144-05709
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetGC
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GC Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2638
  • Target name
    GC
  • Target description
    GC vitamin D binding protein
  • Target synonyms
    DBP, DBP-maf, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDB, VDBG, VDBP, vitamin D-binding protein, epididymis secretory protein Li 51, gc protein-derived macrophage activating factor, gc-globulin, group-specific component (vitamin D binding protein), vitamin D-binding alpha-globulin, vitamin D-binding protein-macrophage activating factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02774
  • Protein Name
    Vitamin D-binding protein
  • Scientific Description
    GC Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Vitamin D Binding protein using anti-Vitamin D Binding protein antibody (A03364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitamin D Binding protein antigen affinity purified polyclonal antibody (Catalog # A03364-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitamin D Binding protein at approximately 53KD. The expected band size for Vitamin D Binding protein is at 53KD.
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