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Western Blot analysis of COS7 cells using DBP Polyclonal Antibody
Western Blot analysis of COS7 cells using DBP Polyclonal Antibody
Western Blot analysis of COS7 cells using DBP Polyclonal Antibody

GC Antibody

CSB-PA002082
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey
TargetGC
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Overview

  • Supplier
    Cusabio
  • Product Name
    GC Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2638
  • Target name
    GC
  • Target description
    GC vitamin D binding protein
  • Target synonyms
    DBP, DBP-maf, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDB, VDBG, VDBP, vitamin D-binding protein, epididymis secretory protein Li 51, gc protein-derived macrophage activating factor, gc-globulin, group-specific component (vitamin D binding protein), vitamin D-binding alpha-globulin, vitamin D-binding protein-macrophage activating factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02774
  • Protein Name
    Vitamin D-binding protein
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Vitamin D Binding protein using anti-Vitamin D Binding protein antibody (A03364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitamin D Binding protein antigen affinity purified polyclonal antibody (Catalog # A03364-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitamin D Binding protein at approximately 53KD. The expected band size for Vitamin D Binding protein is at 53KD.
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