Anti-Gelsolin [GsnVHH 13]

Absolute Antibody

Anti-Gelsolin [GsnVHH 13]

9

This antibody recognizes Ca2+ activated gelsolin. This antibody specifically recognizies and epitope in the G4-G5 domain of gelsolin.

Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds calcium activated gelsolin with a Kd of ~10 nM. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. (PMID:20140750).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA

Research Use Only

Monoclonal

GsnVHH 13

GSN

gelsolin

actin-depolymerizing factor; ADF; AGEL; brevin; epididymis secretory sperm binding protein; gelsolin

Human

IgA

Gelsolin

This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203