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ProductsBack/Anti-Gelsolin [GsnVHH 13]

Anti-Gelsolin [GsnVHH 13]

AB02737-23.159-BT
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGSN
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Gelsolin [GsnVHH 13]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds calcium activated gelsolin with a Kd of ~10 nM. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. (PMID:20140750).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GsnVHH 13
  • Gene ID2934
  • Target name
    GSN
  • Target description
    gelsolin
  • Target synonyms
    ADF, AGEL, AMYLD4, gelsolin, actin-depolymerizing factor, brevin, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06396
  • Protein Name
    Gelsolin
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Gelsolin using anti-Gelsolin antibody (PB9209). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin antigen affinity purified polyclonal antibody (Catalog # PB9209) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 86 kDa. The expected band size for Gelsolin is at 86 kDa.
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