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ProductsBack/Gelsolin Recombinant Antibody
Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Thymus; Lane 2: Mouse Lung; Lane 3: THP-1 Cells; Lane 4: HepG2 Cells; Probed with Gelsolin (2G6) Monoclonal Antibody (bsm-54290R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Gelsolin Recombinant Antibody

BSM-54290R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGSN
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Overview

  • Supplier
    Bioss
  • Product Name
    Gelsolin Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:50-200), IHC-F(IHC-F=1:50-200), IF(IF=1:50-200), ICC/IF(ICC/IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2934
  • Target name
    GSN
  • Target description
    gelsolin
  • Target synonyms
    ADF, AGEL, AMYLD4, gelsolin, actin-depolymerizing factor, brevin, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06396
  • Protein Name
    Gelsolin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Gelsolin using anti-Gelsolin antibody (PB9209). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin antigen affinity purified polyclonal antibody (Catalog # PB9209) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 86 kDa. The expected band size for Gelsolin is at 86 kDa.
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