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Gelsolin Antibody (YA1570, Rabbit)

HY-P81825
MedChem Express
TargetGSN
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Gelsolin Antibody (YA1570, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2934
  • Target name
    GSN
  • Target description
    gelsolin
  • Target synonyms
    ADF, AGEL, AMYLD4, gelsolin, actin-depolymerizing factor, brevin, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Gelsolin Antibody (YA1570) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1570), targeting Gelsolin, with a predicted molecular weight of 86 kDa (observed band size: 86 kDa). Gelsolin Antibody (YA1570) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Gelsolin using anti-Gelsolin antibody (PB9209). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin antigen affinity purified polyclonal antibody (Catalog # PB9209) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 86 kDa. The expected band size for Gelsolin is at 86 kDa.
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