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Anti-Geminin Antibody

R1204-2
HUABIO
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetGMNN
100 ul
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Overview

  • Supplier
    HUABIO
  • Product Name
    Anti-Geminin Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB,IF-Cell,FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51053
  • Target name
    GMNN
  • Target description
    geminin DNA replication inhibitor
  • Target synonyms
    Gem, MGORS6, geminin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75496
  • Protein Name
    Geminin
  • Scientific Description
    Geminin is a nuclear protein that is present in most eukaryotics and highly conserved across species. Numerous functions have been elucidated for Geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. During S phase, geminin is a negative regulator of DNA replication. In many cancer cell lines, inhibition of geminin by RNAi results in re-replication of portions of the genome, which leads to aneuploidy.
  • Reactivity
    Human, Mouse
  • Reactivity Supplier
    Human,Mouse
  • Reactivity Supplier Note
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Geminin/GMNN using anti-Geminin/GMNN antibody (A06060-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Geminin/GMNN antigen affinity purified polyclonal antibody (Catalog # A06060-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Geminin/GMNN at approximately 25 kDa. The expected band size for Geminin/GMNN is at 25 kDa.
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