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Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Anti-GGH Antibody

HPA025226
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetGGH
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GGH
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8836
  • Target name
    GGH
  • Target description
    gamma-glutamyl hydrolase
  • Target synonyms
    gamma-glutamyl hydrolase; gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase); gamma-Glu-X carboxypeptidase; GATD10; GH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92820
  • Protein Name
    Gamma-glutamyl hydrolase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: GGH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
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Figure 1. Western blot analysis of GGH using anti-GGH antibody (A03161-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGH antigen affinity purified polyclonal antibody (Catalog # A03161-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGH at approximately 36 kDa. The expected band size for GGH is at 36 kDa.
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