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Glutamyl hydrolase gamma antibody [N1C3]

GTX109123
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGGH
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Overview

  • Supplier
    GeneTex
  • Product Name
    Glutamyl hydrolase gamma antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.43 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8836
  • Target name
    GGH
  • Target description
    gamma-glutamyl hydrolase
  • Target synonyms
    GATD10, GH, gamma-glutamyl hydrolase, gamma-Glu-X carboxypeptidase, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92820
  • Protein Name
    Gamma-glutamyl hydrolase
  • Scientific Description
    This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kuo WT, Tu DG, Chiu LY, et al. High pemetrexed sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase. Oncol Rep. 2017,38(5):2787-2795. doi: 10.3892/or.2017.5951
    Read this paper
  • Chiu LY, Hsin IL, Yang TY, et al. The ERK-ZEB1 pathway mediates epithelial-mesenchymal transition in pemetrexed resistant lung cancer cells with suppression by vinca alkaloids. Oncogene. 2017,36(2):242-253. doi: 10.1038/onc.2016.195
    Read this paper

Datasheet

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Figure 1. Western blot analysis of GGH using anti-GGH antibody (A03161-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGH antigen affinity purified polyclonal antibody (Catalog # A03161-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGH at approximately 36 kDa. The expected band size for GGH is at 36 kDa.
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