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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: GGH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: GGH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: GGH antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa

GGH Antibody

CSB-PA009389LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGGH
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Overview

  • Supplier
    Cusabio
  • Product Name
    GGH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8836
  • Target name
    GGH
  • Target description
    gamma-glutamyl hydrolase
  • Target synonyms
    gamma-glutamyl hydrolase; gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase); gamma-Glu-X carboxypeptidase; GATD10; GH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92820
  • Protein Name
    Gamma-glutamyl hydrolase
  • Scientific Description
    Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GGH using anti-GGH antibody (A03161-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGH antigen affinity purified polyclonal antibody (Catalog # A03161-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGH at approximately 36 kDa. The expected band size for GGH is at 36 kDa.
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