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Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.

Anti-GIGYF2 Antibody

HPA050899
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetGIGYF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GIGYF2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26058
  • Target name
    GIGYF2
  • Target description
    GRB10 interacting GYF protein 2
  • Target synonyms
    GYF2, PARK11, PERQ2, PERQ3, TNRC15, GRB10-interacting GYF protein 2, PERQ amino acid rich, with GYF domain 3, PERQ amino acid-rich with GYF domain-containing protein 2, Parkinson disease (autosomal recessive, early onset) 11, trinucleotide repeat-containing gene 15 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6Y7W6
  • Protein Name
    GRB10-interacting GYF protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIGYF2 antigen affinity purified polyclonal antibody (Catalog # A04303-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIGYF2 at approximately 180 kDa. The expected band size for GIGYF2 is at 180 kDa.
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