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WB analysis of various sample lysates using GTX66085 GIGYF2 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66085 GIGYF2 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66085 GIGYF2 antibody. Dilution : 1:3000 Loading : 25microg per lane

GIGYF2 antibody

GTX66085
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetGIGYF2
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Overview

  • Supplier
    GeneTex
  • Product Name
    GIGYF2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26058
  • Target name
    GIGYF2
  • Target description
    GRB10 interacting GYF protein 2
  • Target synonyms
    GRB10-interacting GYF protein 2; GYF2; PARK11; Parkinson disease (autosomal recessive, early onset) 11; PERQ amino acid rich, with GYF domain 3; PERQ amino acid-rich with GYF domain-containing protein 2; PERQ2; PERQ3; TNRC15; trinucleotide repeat-containing gene 15 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6Y7W6
  • Protein Name
    GRB10-interacting GYF protein 2
  • Scientific Description
    This gene contains CAG trinucleotide repeats and encodes a protein containing several stretches of polyglutamine residues. The encoded protein may be involved in the regulation of tyrosine kinase receptor signaling. This gene is located in a chromosomal region that was genetically linked to Parkinson disease type 11, and mutations in this gene were thought to be causative for this disease. However, more recent studies in different populations have been unable to replicate this association. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIGYF2 antigen affinity purified polyclonal antibody (Catalog # A04303-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIGYF2 at approximately 180 kDa. The expected band size for GIGYF2 is at 180 kDa.
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