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GIGYF2 Antibody

LS-C747270
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetGIGYF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GIGYF2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 148kDa/149kDa/150kDa/152kDa, while the observed MW by Western blot was 180kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 148kDa/149kDa/150kDa/152kDa, while the observed MW by Western blot was 180kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26058
  • Target name
    GIGYF2
  • Target description
    GRB10 interacting GYF protein 2
  • Target synonyms
    GRB10-interacting GYF protein 2; GYF2; PARK11; Parkinson disease (autosomal recessive, early onset) 11; PERQ amino acid rich, with GYF domain 3; PERQ amino acid-rich with GYF domain-containing protein 2; PERQ2; PERQ3; TNRC15; trinucleotide repeat-containing gene 15 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIGYF2 using anti-GIGYF2 antibody (A04303-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIGYF2 antigen affinity purified polyclonal antibody (Catalog # A04303-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIGYF2 at approximately 180 kDa. The expected band size for GIGYF2 is at 180 kDa.
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