Anti-GITR [2D3]

Absolute Antibody

Anti-GITR [2D3]

7

This antibody binds human and rhesus monkey GITR (TNFRSF18; CD357) and does not cross react with mouse GITR. This protein acts as a receptor for TNFSF18, which is involved in interactions between activated T-lymphocytes and endothelial cells and in the re

The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey GITR was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey GITR and not mouse GITR. The binding affinity of the original mouse IgG1 antibody for human GITR was determined using surface plasmon resonance and was found to be Kd= 7.75X 10^(-10)M. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hGITR mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).

Flow Cytometry, ELISA

ELISA; FC; therapeutic

Research Use Only

Monoclonal

2D3

TNFRSF18

TNF receptor superfamily member 18

activation-inducible TNFR family receptor; AITR; CD357; ENERGEN; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; tumor necrosis factor receptor superfamily member 18

Rabbit

IgG

Tumor necrosis factor receptor superfamily member 18

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Primate

Human, rhesus monkey

The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human GITR protein.

-20°C,2°C to 8°C

12352203