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Figure 1. Western blotting validation for Anti-GLDC Antibody A04777 Western blot analysis of extracts of various cells
Figure 1. Western blotting validation for Anti-GLDC Antibody A04777 Western blot analysis of extracts of various cells
Figure 1. Western blotting validation for Anti-GLDC Antibody A04777 Western blot analysis of extracts of various cells

Anti-GLDC Antibody

A04777
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGLDC
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GLDC Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID2731
  • Target name
    GLDC
  • Target description
    glycine decarboxylase
  • Target synonyms
    GCE, GCE1, GCSP, HYGN1, glycine dehydrogenase (decarboxylating), mitochondrial, glycine cleavage system protein P, glycine decarboxylase P-protein, glycine dehydrogenase (aminomethyl-transferring), glycine dehydrogenase [decarboxylating], mitochondrial, nonketotic hyperglycinemia
  • Host
    Rabbit
  • Protein IDP23378
  • Protein Name
    Glycine dehydrogenase (decarboxylating), mitochondrial
  • Scientific Description
    Boster Bio Anti-GLDC Antibody catalog # A04777. Tested in WB,IHC,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycine decarboxylase/GLDC using anti-Glycine decarboxylase/GLDC antibody (A04777-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycine decarboxylase/GLDC antigen affinity purified polyclonal antibody (Catalog # A04777-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycine decarboxylase/GLDC at approximately 113KD. The expected band size for Glycine decarboxylase/GLDC is at 113KD.
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Boster Bio
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ReactivityHuman, Mouse, Rat
TargetGLDC
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TargetGLDC
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IHC image of CSB-PA328400LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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TargetGLDC
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA002318 antibody. Corresponding GLDC RNA-seq data are presented for the same tissues.
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TargetGLDC
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Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267). Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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TargetGLDC
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