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Anti-GOLGA2 Antibody

144-05344
RayBiotech
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetGOLGA2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GOLGA2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.96 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2801
  • Target name
    GOLGA2
  • Target description
    golgin A2
  • Target synonyms
    130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; golgin subfamily A member 2; golgin-95; SY11 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08379
  • Protein Name
    Golgin subfamily A member 2
  • Scientific Description
    GOLGA2 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GM130 using anti-GM130 antibody (A05865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM130 antigen affinity purified polyclonal antibody (Catalog # A05865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130 kDa. The expected band size for GM130 is at 113 kDa.
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