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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA146152(GOLGA2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA146152(GOLGA2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA146152(GOLGA2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)

GOLGA2 Antibody

CSB-PA146152
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGOLGA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GOLGA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2801
  • Target name
    GOLGA2
  • Target description
    golgin A2
  • Target synonyms
    130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; golgin subfamily A member 2; golgin-95; SY11 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08379
  • Protein Name
    Golgin subfamily A member 2
  • Scientific Description
    The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GM130 using anti-GM130 antibody (A05865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM130 antigen affinity purified polyclonal antibody (Catalog # A05865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130 kDa. The expected band size for GM130 is at 113 kDa.
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