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Immunohistochemical staining of human gastrointestinal, placenta, prostate and squamous epithelia using Anti-GOLGA2 antibody HPA021178 (A) shows similar protein distribution across tissues to independent antibody HPA021799 (B).
Immunohistochemical staining of human gastrointestinal, placenta, prostate and squamous epithelia using Anti-GOLGA2 antibody HPA021178 (A) shows similar protein distribution across tissues to independent antibody HPA021799 (B).
Immunohistochemical staining of human gastrointestinal, placenta, prostate and squamous epithelia using Anti-GOLGA2 antibody HPA021178 (A) shows similar protein distribution across tissues to independent antibody HPA021799 (B).

Anti-GOLGA2 Antibody

HPA021178
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGOLGA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GOLGA2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2801
  • Target name
    GOLGA2
  • Target description
    golgin A2
  • Target synonyms
    DEDHMB, GM130, golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, Golgi matrix protein GM130, SY11 protein, golgi autoantigen, golgin subfamily a, 2, golgin-95
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08379
  • Protein Name
    Golgin subfamily A member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of GM130 using anti-GM130 antibody (A05865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM130 antigen affinity purified polyclonal antibody (Catalog # A05865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130 kDa. The expected band size for GM130 is at 113 kDa.
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