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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Anti-GOPC Antibody

HPA024018
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGOPC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GOPC
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57120
  • Target name
    GOPC
  • Target description
    golgi associated PDZ and coiled-coil motif containing
  • Target synonyms
    CAL, FIG, GOPC1, PIST, dJ94G16.2, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, PDZ protein interacting specifically with TC10, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, dJ94G16.2 PIST, fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HD26
  • Protein Name
    Golgi-associated PDZ and coiled-coil motif-containing protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIST/GOPC antigen affinity purified polyclonal antibody (Catalog # A03660-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIST/GOPC at approximately 55 kDa. The expected band size for PIST/GOPC is at 55 kDa.
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