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Immunohistochemistry analysis in human heart muscle and endometrium tissues using HPA072629 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human heart muscle and endometrium tissues using HPA072629 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human heart muscle and endometrium tissues using HPA072629 antibody. Corresponding GOT1 RNA-seq data are presented for the same tissues.

Anti-GOT1-25ul

HPA072629
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GOT1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB, ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17174
  • Protein Name
    Aspartate aminotransferase, cytoplasmic
  • Scientific Description
    Rabbit Polyclonal Anti-GOT1 Antibody against Human glutamic-oxaloacetic transaminase 1, soluble. Validated for Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (A04085-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aspartate Aminotransferase/GOT1 antigen affinity purified polyclonal antibody (Catalog # A04085-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aspartate Aminotransferase/GOT1 at approximately 41KD. The expected band size for Aspartate Aminotransferase/GOT1 is at 46KD.
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